Estrogenic effects of marijuana smoke
condensate and cannabinoid compounds
by
Lee SY, Oh SM, Chung KH.
National Institute of Scientific Investigation,
331-1 Shinwol-7-dong, Yangcheon-gu,
Seoul 158-707, Korea;
Sungkyunkwan University,
300 Cheoncheon-dong, Jangan-gu,
Suwon, Gyeonggi-do 440-746, Korea.
Toxicol Appl Pharmacol. 2006 Feb 22;
ABSTRACTChronic exposure to marijuana produces adverse effects on the endocrine and reproductive systems in humans; however, the experimental evidence for this presented thus far has not been without controversy. In this study, the estrogenic effect of marijuana smoke condensate (MSC) was evaluated using in vitro bioassays, viz., the cell proliferation assay, the reporter gene assay, and the ER competitive binding assay. The results of these assays were compared with those of three major cannabinoids, i.e., THC, CBD, and CBN. The estrogenic effect of MSC was further confirmed by the immature female rat uterotrophic assay. MSC stimulated the estrogenicity related to the ER-mediated pathway, while neither THC, CBD, nor CBN did. Moreover, treatment with 10 and 25 mg/kg MSC induced significant uterine response, and 10 mg/kg MSC resulted in an obvious change in the uterine epithelial cell appearance. MSC also enhanced the IGFBP-1 gene expression in a dose-dependent manner. To identify the constituents of MSC responsible for its estrogenicity, the MSC fractionated samples were examined using another cell proliferation assay, and the estrogenic active fraction was analyzed using GC-MS. In the organic acid fraction that showed the strongest estrogenic activity among the seven fractions of MSC, phenols were identified. Our results suggest that marijuana abuse is considered an endocrine-disrupting factor. Furthermore, these results suggest that the phenolic compounds contained in MSC play a role in its estrogenic effect.THC
Bliss
Aerosol
Memory
Anandamide
Just say know
Tolerant mice
Forgetful rodents
Endocannabinoids
Stoned as a newt?
The nectar of delight01 02 03 04 05 06 07 08 09 10 11 12 13 14 15
Refs
HedWeb
cocaine.wiki
Future Opioids
BLTC Research
Paradise-Engineering
The Hedonistic Imperative
MDMA: Utopian Pharmacology
The Good Drug Guide
The Responsible Parent's Guide
To Healthy Mood Boosters For All The Family
